Research & Development
We pride ourselves on our strong capability to be innovative and always challenge the existing paradigms. Our in-house R&D programs are committed to innovating and advancing new strategies that tackle the technical challenges commonly present in the development, analysis, and application of organoids.
Our primary goal is to deliver a standardized, easily integrated system featuring efficient and scalable processes and assay pipelines, enabling precise, high-resolution and quantitative characterization of organoid cell heterogeneity with medium- to high-throughput efficiency. We expect our invention will fill a critical gap in organoid research and application by offering a robust approach to evaluate and monitor the quality of diverse organoid types and their responses to stimuli, simultaneously across multiple individuals.
Ultimately, we like to establish an industry quality-control standard for manufacturing hPSC-derived organoids and to develop digital organoid models aiding to the use of organoids in future medicine.
Cell reprogramming & differentiation
Using four reprogramming factors, we can drive terminally differentiated cells to a pluripotent stage, creating induced pluripotent stem cells (iPSCs).
Human iPSCs (hiPSCs) can differentiate into nearly all functional somatic cell types. In organoid systems, hiPSC differentiation enables the co-development of various cell types that form specific tissues. hiPSC-derived organoids are essentially a result of guided hiPSC differentiation under 3D conditions.
Cell heterogeneities in iPSC-derived organoids
Cell heterogeneity drives biological complexity essential for mammalian organ functionality. Cells show significant molecular diversity within the same organ and across individuals, influencing their normal function, disease manifestation, and therapeutic responses.
As cellular diversity increases during organoid development, effectively defining cell heterogeneity is crucial for establishing quality-control standards across different developmental stages, individuals, and stimuli. This is key for the reliable use of organoids in disease modeling and therapy development.
Single-cell analysis
Single-cell analysis is a powerful approach that permits in-depth studies of individual cells within complex populations. It provides critical resolution to delineate cell heterogeneity in tissues or organoids.
Based on several recent advancements for gene expression profiling techniques with single-cell resolution, OrganoidPro R&D is pushing the boundary of organoid studies by developing proprietary methods and tools for quantitatively profiling heterogeneous cells within organoids derived from different individuals at an easily scalable throughput.
Digital twins of organoids
The major progress in big data analysis and generative AI fields provides us with exciting opportunities for creating comprehensive digital models that could well simulate complex systems in the real-world.
Leveraging the large quantity of unique cell heterogeneity data obtained from our internal R&D and external services, we also work on using machine learning techniques to develop "digital twins" that accurately model and predict cellular and molecular responses of organoids to specific stimuli in silico.